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Isoleucine and Leucine: study protocols

14 study groups took Isoleucine together with Leucine. These are the doses the studies gave. They are not a recommendation.

Isoleucine: what the research found →

Dose per serving from research: Isoleucine

7 study groups in 1 study say how much Isoleucine participants took a day.

These are the doses the studies gave. They are not a recommendation.

Most common: 50 mg/kg to 100 mg/kg, 71% of 7 study groups.

Daily doseStudy groups
10 mg/kg to 25 mg/kg
2
25 mg/kg to 50 mg/kg
0
50 mg/kg to 100 mg/kg
5

Dose per serving from research: Leucine

12 study groups in 6 studies say how much Leucine participants took a day.

These are the doses the studies gave. They are not a recommendation.

Most common: 10 mg to 25 mg, 33% of 12 study groups.

Daily doseStudy groups
10 mg or less
1
10 mg to 25 mg
4
25 mg to 50 mg
2
50 mg to 100 mg
0
100 mg to 250 mg
0
250 mg to 500 mg
0
500 mg to 1 g
0
1 g to 2.5 g
0
2.5 g to 5 g
3
5 g to 10 g
0
10 g to 25 g
2

Commonly used together

14 of 14 study groups took Isoleucine and Leucine with something else. Shown: what 3 or more different studies gave.

What was given together, not what works together.

Taken withStudy groups
Branched-Chain Amino Acids
9
Valine
4

14 study groups

Study
Anti-sarcopenic effect of leucine-enriched branched-chain amino acid supplementation among elderly chronic kidney disease patients: a double-blinded randomized controlled trial
2025 · oral mixed BCAA supplementation · Patients with chronic kidney disease aged over 65 years
1.5 g4.5 g12 weeks29
Post-exercise branched chain amino acid supplementation does not affect recovery markers following three consecutive high intensity resistance training bouts compared to carbohydrate supplementation
2016 · BCAA-CHO · Resistance-trained, apparently healthy males; mean age 21.8 years
1 g3 g4 days15
Acute oral administration of a tyrosine and phenylalanine-free amino acid mixture reduces exercise capacity in the heat
2013 · TYR-free · Healthy, moderately trained men unacclimated to exercise in the heat
15 g22.5 g1 day8
Acute oral administration of a tyrosine and phenylalanine-free amino acid mixture reduces exercise capacity in the heat
2013 · BAL · Healthy, moderately trained men unacclimated to exercise in the heat
15 g22.5 g1 day8
IPP-rich milk protein hydrolysate lowers blood pressure in subjects with stage 1 hypertension, a randomized controlled trial
2010 · MPH2 · Caucasian men and women with stage 1 hypertension or prehypertension; mean age 58 ± 8 years
3.6 mg4.6 mg4 weeks70
Attenuation of the protein wasting associated with bed rest by branched-chain amino acids
1999 · BCAA group · Healthy subjects undergoing bed rest
30 mmol30 mmol6 days8
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 1: leucine 40 mg/kg/day, valine 80 mg/kg/day, isoleucine 62 mg/kg/day · Healthy young adult men; six subjects were in phase 1
62 mg/kg40 mg5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 1: leucine 15 mg/kg/day, valine 80 mg/kg/day, isoleucine 62 mg/kg/day · Healthy young adult men; six subjects were in phase 1
62 mg/kg15 mg5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 1: leucine 40 mg/kg/day, valine 20 mg/kg/day, isoleucine 62 mg/kg/day · Healthy young adult men; six subjects were in phase 1
62 mg/kg—5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 1: leucine 15 mg/kg/day, valine 20 mg/kg/day, isoleucine 62 mg/kg/day · Healthy young adult men; six subjects were in phase 1
62 mg/kg15 mg5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 2: leucine 15 mg/kg/day, valine 20 mg/kg/day, isoleucine 62 mg/kg/day · Healthy young adult men; five subjects were in phase 2
62 mg/kg15 mg5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 2: leucine 40 mg/kg/day, valine 20 mg/kg/day, isoleucine 20 mg/kg/day · Healthy young adult men; five subjects were in phase 2
20 mg/kg40 mg5 days—
Branched-chain amino acid interactions with reference to amino acid requirements in adult men: leucine metabolism at different valine and isoleucine intakes
1991 · Phase 2: leucine 15 mg/kg/day, valine 20 mg/kg/day, isoleucine 20 mg/kg/day · Healthy young adult men; five subjects were in phase 2
20 mg/kg15 mg5 days—
Dileucine-supplemented essential amino acids support whole-body anabolism after resistance exercise and serum-stimulated cell-based anabolism
2025 · Branched-chain amino acid (BCAA) supplement · Healthy recreationally active adults, 27.0 ± 4.7 years old; 8 men and 4 women
1.5 g3.1 g1 day12
Isoleucine and Leucine: study protocols | supp.fit