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Folic Acid and Vitamin D: study protocols

16 study groups took Folic Acid together with Vitamin D. These are the doses the studies gave. They are not a recommendation.

Folic Acid: what the research found →

Dose per serving from research: Folic Acid

16 study groups in 13 studies say how much Folic Acid participants took a day.

These are the doses the studies gave. They are not a recommendation.

Most common: 250 mcg to 500 mcg, 75% of 16 study groups.

Daily doseStudy groups
100 mcg or less
1
100 mcg to 250 mcg
1
250 mcg to 500 mcg
12
500 mcg to 1 mg
2

Dose per serving from research: Vitamin D

8 study groups in 5 studies say how much Vitamin D participants took a day.

These are the doses the studies gave. They are not a recommendation.

Most common: 100 IU to 250 IU, 50% of 8 study groups.

Daily doseStudy groups
100 IU or less
1
100 IU to 250 IU
4
250 IU to 500 IU
3

Commonly used together

16 of 16 study groups took Folic Acid and Vitamin D with something else. Shown: what 3 or more different studies gave. The 10 most common of 14.

What was given together, not what works together.

Taken withStudy groups
Iron
14
Vitamin A
14
Vitamin C
14
Vitamin E
14
Zinc
14
Copper
13
Iodine
13
Vitamin B12
13
Niacin
12
Selenium
12

16 study groups

Study
Impact of micronutrient supplementation during pregnancy on birth weight, duration of gestation, and perinatal mortality in rural western China: double blind cluster randomised controlled trial
2008 · Multiple micronutrients · Pregnant women resident in disadvantaged rural counties in Shaanxi Province, China; eligible women enrolled before 28 weeks’ gestation
400 mcg5 mcg—1,899
Equity in adherence to and effect of prenatal food and micronutrient supplementation on child mortality: results from the MINIMat randomized trial, Bangladesh
2014 · EMMS (early food invitation; multiple micronutrients) · Pregnant women in rural Bangladesh, enrolled before 14 weeks’ gestation
400 mcg200 IU——
Equity in adherence to and effect of prenatal food and micronutrient supplementation on child mortality: results from the MINIMat randomized trial, Bangladesh
2014 · UMMS (usual food invitation; multiple micronutrients) · Pregnant women in rural Bangladesh, enrolled before 14 weeks’ gestation
400 mcg200 IU——
Modifying effects of maternal Hb concentration on infant birth weight in women receiving prenatal iron-containing supplements: a randomised controlled trial
2016 · MMN group · Pregnant women aged at least 20 years, nulliparous, with Hb concentration ≥100 g/L and enrolled before 20 weeks of gestation
400 mcg5 mcg——
Effects of maternal micronutrient supplementation on fetal loss and infant mortality: a cluster-randomized trial in Nepal
2003 · Multiple micronutrients · Pregnant women in rural Nepal
400 mcg10 mcg——
Predictors of adherence to micronutrient supplementation before and during pregnancy in Vietnam
2017 · MM (multiple micronutrients) preconception group · Women of reproductive age in Vietnam intending to become pregnant
400 mcg85.7 IU54 weeks1,469
Assessing the adherence and acceptability to iron and folic acid compared with multiple micronutrient supplements during pregnancy: a cluster-randomized noninferiority trial in Cambodia
2025 · MMS-180 · Healthy pregnant individuals aged 18–45 years, in the first 14 weeks of a low-risk singleton pregnancy
400 mcg200 IU180 days516
Assessing the adherence and acceptability to iron and folic acid compared with multiple micronutrient supplements during pregnancy: a cluster-randomized noninferiority trial in Cambodia
2025 · MMS-90 · Healthy pregnant individuals aged 18–45 years, in the first 14 weeks of a low-risk singleton pregnancy
400 mcg200 IU180 days515
A 2-Year Randomized Controlled Trial With Low-Dose B-Vitamin Supplementation Shows Benefits on Bone Mineral Density in Adults With Lower B12 Status
2022 · B-vitamin treatment · Community-dwelling adults aged 50 years or older; men and women; no specific diagnosed condition, and not currently taking B-vitamin supplements at screening
200 mcg10 mcg2 years103
Maternal B-vitamin and vitamin D status before, during, and after pregnancy and the influence of supplementation preconception and during pregnancy: Prespecified secondary analysis of the NiPPeR double-blind randomized controlled trial
2023 · intervention · Women planning pregnancy; generally healthy, recruited before conception
400 mcg10 mcg—870